Rayners Lane FC was originally founded in Harrow, Middlesex, in 1933 and spent their formative years playing in local football. By the early 1960’s they had joined the Spartan League, a tenth spot finish in 1966-67 their highest placing. In 1978-79 they had joined the Hellenic League and they were crowned Division 1 champions on goal difference in 1982-83,earning promotion to the Hellenic Premier Division where they stayed for eleven seasons before lack of floodlights saw the club returned to Division 1. Two seasons in Hellenic Division 1 were followed by two seasons in the Middlesex League before they joined the Chiltonian League Premier Division in 1998-99.

The Chiltonian and Hellenic Leagues merged in 2000-01 and Rayners Lane found themselves in Division 1 East which they won in 2012-13. However, they were denied promotion due to ground grading issues and stayed in Division 1 until the 2017-18 season when they moved to the Spartan South Midlands Division 1. That summer work began on installing a 3G playing surface along with a new club house and the Tithe Farm Sports & Social Club was eventually opened in October 2019. A year later long time club stalwart Tony Pratt was recognized by the FA with Volunteer Of The Year Award for over 50 years of service, a fine achievement for both the club and Tony.

The club remained in the Spartan South Midlands League until the 2021-22 campaign when restructuring by the FA saw them moved over to the Combined Counties League Division 1.A fourth place finish saw them reach the Play-Offs where they lost in the semi-final to eventual winners Wallingford Town. The following season saw them again finish fourth which resulted in another Play-Off spot where this time they overcame London Samurai Rovers in the semi-final and then Langley in the final to earn promotion. They also beat British Airways FC to win the Middlesex Premier Cup to chalk up a first ever League and Cup double.

2023-24 sees Rayners Lane competing in the Combined Counties Premier Division North under the stewardship of Gary Meakin and Wes Daly as they attempt to bring Step 4football to The Lane for the first time ever.